pBY011

DNASU
Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.

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Cutters
Show ORFs
Cutters
From: 222 To: 555 Length: 334bp Melting Temp: 63.1 °C GC Content: 55%
Name Type Location Length
ColE Bacterial Origin 3651 to 4333 683 bp
ccdB CDS 5124 to 4915 210 bp
M13 forward Other 7200 to 7183 18 bp
M13 reverse Other 4702 to 4722 21 bp
Gal1 Promoter 7145 to 6934 212 bp
Ura3 promoter Promoter 2378 to 2349 30 bp
attR1 Recombination Site 6439 to 6455 17 bp
attR2 Recombination Site 4749 to 4764 16 bp
AmpR Antibiotic Resistance 2894 to 3553 660 bp
CmR Antibiotic Resistance 6222 to 5566 657 bp
Ura3 Antibiotic Resistance 2263 to 1460 804 bp
CEN Protein Binding Site 255 to 7396 7142 bp
- / - - Location: - Length: -

Manage Enzymes

Enzyme Cuts
AatII 0
Acc65I 0
AccI 0
AciI 0
AclI 0
AcuI 0
AfeI 0
AflII 0
AflIII 0
AgeI 0
AhdI 0
AleI 0
AluI 0
AlwI 0
AlwNI 0
ApaI 0
ApaLI 0
ApeKI 0
ApoI 0
AscI 0
AseI 0
AsiSI 0
AvaI 0
AvaII 0
AvrII 0
BaeGI 0
BaeI 0
BamHI 0
BanI 0
BanII 0
BbsI 0
BbvCI 0
BbvI 0
BccI 0
BceAI 0
BcgI 0
BciVI 0
BclI 0
BfaI 0
BfuAI 0
BfuCI 0
BglI 0
BglII 0
BlpI 0
BmgBI 0
BmrI 0
BmtI 0
BpmI 0
Bpu10I 0
BpuEI 0
BsaAI 0
BsaBI 0
BsaHI 0
BsaI 0
BsaJI 0
BsaWI 0
BsaXI 0
BseRI 0
BseYI 0
BsgI 0
BsiEI 0
BsiHKAI 0
BsiWI 0
BslI 0
BsmAI 0
BsmBI 0
BsmFI 0
BsmI 0
BsoBI 0
Bsp1286I 0
BspCNI 0
BspDI 0
BspEI 0
BspHI 0
BspMI 0
BspQI 0
BsrBI 0
BsrDI 0
BsrFI 0
BsrGI 0
BsrI 0
BssHII 0
BssKI 0
BssSI 0
BstAPI 0
BstBI 0
BstEII 0
BstNI 0
BstUI 0
BstXI 0
BstYI 0
BstZ17I 0
Bsu36I 0
BtgI 0
BtgZI 0
BtsCI 0
BtsI 0
Cac8I 0
ClaI 0
CspCI 0
CviAII 0
CviKI_1 0
CviQI 0
DdeI 0
DpnI 0
DpnII 0
DraI 0
DraIII 0
DrdI 0
EaeI 0
EagI 0
EarI 0
EciI 0
Eco53kI 0
EcoNI 0
EcoO109I 0
EcoRI 0
EcoRV 0
FatI 0
FauI 0
Fnu4HI 0
FokI 0
FseI 0
FspI 0
HaeII 0
HaeIII 0
HgaI 0
HhaI 0
HinP1I 0
HincII 0
HindIII 0
HinfI 0
HpaI 0
HpaII 0
HphI 0
Hpy166II 0
Hpy188I 0
Hpy188III 0
Hpy99I 0
HpyAV 0
HpyCH4III 0
HpyCH4IV 0
HpyCH4V 0
KasI 0
KpnI 0
MboI 0
MboII 0
MfeI 0
MluI 0
MlyI 0
MmeI 0
MnlI 0
MscI 0
MseI 0
MslI 0
MspA1I 0
MspI 0
MwoI 0
NaeI 0
NarI 0
NciI 0
NcoI 0
NdeI 0
NgoMIV 0
NheI 0
NlaIII 0
NlaIV 0
NmeAIII 0
NotI 0
NruI 0
NsiI 0
NspI 0
PacI 0
PaeR7I 0
PciI 0
PflFI 0
PflMI 0
PhoI 0
PleI 0
PmeI 0
PmlI 0
PpuMI 0
PshAI 0
PsiI 0
PspGI 0
PspOMI 0
PspXI 0
PstI 0
PvuI 0
PvuII 0
RsaI 0
RsrII 0
SacI 0
SacII 0
SalI 0
SapI 0
Sau3AI 0
Sau96I 0
SbfI 0
ScaI 0
ScrFI 0
SexAI 0
SfaNI 0
SfcI 0
SfiI 0
SfoI 0
SgrAI 0
SmaI 0
SmlI 0
SnaBI 0
SpeI 0
SphI 0
SspI 0
StuI 0
StyD4I 0
StyI 0
SwaI 0
TaqI 0
TfiI 0
TliI 0
TseI 0
Tsp45I 0
Tsp509I 0
TspMI 0
TspRI 0
Tth111I 0
XbaI 0
XcmI 0
XhoI 0
XmaI 0
XmnI 0
ZraI 0

Restriction Digest

One
Enzyme Cuts
None selected
Two
Enzyme Cuts
None selected
Three
Enzyme Cuts
None selected