pJP1563

DNASU
Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.

Map Viewer

Cutters
Show ORFs
Cutters
From: 222 To: 555 Length: 334bp Melting Temp: 63.1 °C GC Content: 55%
Name Type Location Length
gag/pol/env CDS 1871 to 2644 774 bp
PBSQ Construction Prefix 1140 to 1156 17 bp
bacterial Promoter 3803 to 3677 127 bp
LoxP Recombination Site 3651 to 3684 34 bp
BlastR Antibiotic Resistance 2428 to 2823 396 bp
AmpR Antibiotic Resistance 7094 to 6237 858 bp
5p LTR Other 550 to 1139 590 bp
3p LTR Other 3962 to 4551 590 bp
- / - - Location: - Length: -

Manage Enzymes

Enzyme Cuts
AatII 0
Acc65I 0
AccI 0
AciI 0
AclI 0
AcuI 0
AfeI 0
AflII 0
AflIII 0
AgeI 0
AhdI 0
AleI 0
AluI 0
AlwI 0
AlwNI 0
ApaI 0
ApaLI 0
ApeKI 0
ApoI 0
AscI 0
AseI 0
AsiSI 0
AvaI 0
AvaII 0
AvrII 0
BaeGI 0
BaeI 0
BamHI 0
BanI 0
BanII 0
BbsI 0
BbvCI 0
BbvI 0
BccI 0
BceAI 0
BcgI 0
BciVI 0
BclI 0
BfaI 0
BfuAI 0
BfuCI 0
BglI 0
BglII 0
BlpI 0
BmgBI 0
BmrI 0
BmtI 0
BpmI 0
Bpu10I 0
BpuEI 0
BsaAI 0
BsaBI 0
BsaHI 0
BsaI 0
BsaJI 0
BsaWI 0
BsaXI 0
BseRI 0
BseYI 0
BsgI 0
BsiEI 0
BsiHKAI 0
BsiWI 0
BslI 0
BsmAI 0
BsmBI 0
BsmFI 0
BsmI 0
BsoBI 0
Bsp1286I 0
BspCNI 0
BspDI 0
BspEI 0
BspHI 0
BspMI 0
BspQI 0
BsrBI 0
BsrDI 0
BsrFI 0
BsrGI 0
BsrI 0
BssHII 0
BssKI 0
BssSI 0
BstAPI 0
BstBI 0
BstEII 0
BstNI 0
BstUI 0
BstXI 0
BstYI 0
BstZ17I 0
Bsu36I 0
BtgI 0
BtgZI 0
BtsCI 0
BtsI 0
Cac8I 0
ClaI 0
CspCI 0
CviAII 0
CviKI_1 0
CviQI 0
DdeI 0
DpnI 0
DpnII 0
DraI 0
DraIII 0
DrdI 0
EaeI 0
EagI 0
EarI 0
EciI 0
Eco53kI 0
EcoNI 0
EcoO109I 0
EcoRI 0
EcoRV 0
FatI 0
FauI 0
Fnu4HI 0
FokI 0
FseI 0
FspI 0
HaeII 0
HaeIII 0
HgaI 0
HhaI 0
HinP1I 0
HincII 0
HindIII 0
HinfI 0
HpaI 0
HpaII 0
HphI 0
Hpy166II 0
Hpy188I 0
Hpy188III 0
Hpy99I 0
HpyAV 0
HpyCH4III 0
HpyCH4IV 0
HpyCH4V 0
KasI 0
KpnI 0
MboI 0
MboII 0
MfeI 0
MluI 0
MlyI 0
MmeI 0
MnlI 0
MscI 0
MseI 0
MslI 0
MspA1I 0
MspI 0
MwoI 0
NaeI 0
NarI 0
NciI 0
NcoI 0
NdeI 0
NgoMIV 0
NheI 0
NlaIII 0
NlaIV 0
NmeAIII 0
NotI 0
NruI 0
NsiI 0
NspI 0
PacI 0
PaeR7I 0
PciI 0
PflFI 0
PflMI 0
PhoI 0
PleI 0
PmeI 0
PmlI 0
PpuMI 0
PshAI 0
PsiI 0
PspGI 0
PspOMI 0
PspXI 0
PstI 0
PvuI 0
PvuII 0
RsaI 0
RsrII 0
SacI 0
SacII 0
SalI 0
SapI 0
Sau3AI 0
Sau96I 0
SbfI 0
ScaI 0
ScrFI 0
SexAI 0
SfaNI 0
SfcI 0
SfiI 0
SfoI 0
SgrAI 0
SmaI 0
SmlI 0
SnaBI 0
SpeI 0
SphI 0
SspI 0
StuI 0
StyD4I 0
StyI 0
SwaI 0
TaqI 0
TfiI 0
TliI 0
TseI 0
Tsp45I 0
Tsp509I 0
TspMI 0
TspRI 0
Tth111I 0
XbaI 0
XcmI 0
XhoI 0
XmaI 0
XmnI 0
ZraI 0

Restriction Digest

One
Enzyme Cuts
None selected
Two
Enzyme Cuts
None selected
Three
Enzyme Cuts
None selected