pMCSG11

DNASU
Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).

Map Viewer

Cutters
Show ORFs
Cutters
From: 222 To: 555 Length: 334bp Melting Temp: 63.1 °C GC Content: 55%
Name Type Location Length
MCS2 DNA Linker 2171 to 2216 46 bp
MCS DNA Linker 1801 to 1880 80 bp
LIC DNA Linker 353 to 384 32 bp
ori Bacterial Origin 1821 to 2733 913 bp
Duet Down1 Construction Prefix 208 to 189 20 bp
Duet P1 Construction Prefix 4020 to 4039 20 bp
Duet P2 Construction Prefix 189 to 208 20 bp
T7 Promoter 214 to 230 17 bp
T7 2 Promoter 4063 to 4079 17 bp
TEV Construction Suffix 345 to 365 21 bp
lacO2 Construction Suffix 230 to 235 6 bp
lacO1 Construction Suffix 5 to 25 21 bp
lacI Regulatory Protein 3939 to 2857 1083 bp
RBS2 Other 286 to 291 6 bp
RBS1 Other 58 to 63 6 bp
CmR Antibiotic Resistance 1459 to 800 660 bp
His Signalling Peptide 83 to 100 18 bp
His 2 Signalling Peptide 303 to 320 18 bp
S-tag Signalling Peptide 437 to 481 45 bp
T7 term Terminator 533 to 580 48 bp
- / - - Location: - Length: -

Manage Enzymes

Enzyme Cuts
AatII 0
Acc65I 0
AccI 0
AciI 0
AclI 0
AcuI 0
AfeI 0
AflII 0
AflIII 0
AgeI 0
AhdI 0
AleI 0
AluI 0
AlwI 0
AlwNI 0
ApaI 0
ApaLI 0
ApeKI 0
ApoI 0
AscI 0
AseI 0
AsiSI 0
AvaI 0
AvaII 0
AvrII 0
BaeGI 0
BaeI 0
BamHI 0
BanI 0
BanII 0
BbsI 0
BbvCI 0
BbvI 0
BccI 0
BceAI 0
BcgI 0
BciVI 0
BclI 0
BfaI 0
BfuAI 0
BfuCI 0
BglI 0
BglII 0
BlpI 0
BmgBI 0
BmrI 0
BmtI 0
BpmI 0
Bpu10I 0
BpuEI 0
BsaAI 0
BsaBI 0
BsaHI 0
BsaI 0
BsaJI 0
BsaWI 0
BsaXI 0
BseRI 0
BseYI 0
BsgI 0
BsiEI 0
BsiHKAI 0
BsiWI 0
BslI 0
BsmAI 0
BsmBI 0
BsmFI 0
BsmI 0
BsoBI 0
Bsp1286I 0
BspCNI 0
BspDI 0
BspEI 0
BspHI 0
BspMI 0
BspQI 0
BsrBI 0
BsrDI 0
BsrFI 0
BsrGI 0
BsrI 0
BssHII 0
BssKI 0
BssSI 0
BstAPI 0
BstBI 0
BstEII 0
BstNI 0
BstUI 0
BstXI 0
BstYI 0
BstZ17I 0
Bsu36I 0
BtgI 0
BtgZI 0
BtsCI 0
BtsI 0
Cac8I 0
ClaI 0
CspCI 0
CviAII 0
CviKI_1 0
CviQI 0
DdeI 0
DpnI 0
DpnII 0
DraI 0
DraIII 0
DrdI 0
EaeI 0
EagI 0
EarI 0
EciI 0
Eco53kI 0
EcoNI 0
EcoO109I 0
EcoRI 0
EcoRV 0
FatI 0
FauI 0
Fnu4HI 0
FokI 0
FseI 0
FspI 0
HaeII 0
HaeIII 0
HgaI 0
HhaI 0
HinP1I 0
HincII 0
HindIII 0
HinfI 0
HpaI 0
HpaII 0
HphI 0
Hpy166II 0
Hpy188I 0
Hpy188III 0
Hpy99I 0
HpyAV 0
HpyCH4III 0
HpyCH4IV 0
HpyCH4V 0
KasI 0
KpnI 0
MboI 0
MboII 0
MfeI 0
MluI 0
MlyI 0
MmeI 0
MnlI 0
MscI 0
MseI 0
MslI 0
MspA1I 0
MspI 0
MwoI 0
NaeI 0
NarI 0
NciI 0
NcoI 0
NdeI 0
NgoMIV 0
NheI 0
NlaIII 0
NlaIV 0
NmeAIII 0
NotI 0
NruI 0
NsiI 0
NspI 0
PacI 0
PaeR7I 0
PciI 0
PflFI 0
PflMI 0
PhoI 0
PleI 0
PmeI 0
PmlI 0
PpuMI 0
PshAI 0
PsiI 0
PspGI 0
PspOMI 0
PspXI 0
PstI 0
PvuI 0
PvuII 0
RsaI 0
RsrII 0
SacI 0
SacII 0
SalI 0
SapI 0
Sau3AI 0
Sau96I 0
SbfI 0
ScaI 0
ScrFI 0
SexAI 0
SfaNI 0
SfcI 0
SfiI 0
SfoI 0
SgrAI 0
SmaI 0
SmlI 0
SnaBI 0
SpeI 0
SphI 0
SspI 0
StuI 0
StyD4I 0
StyI 0
SwaI 0
TaqI 0
TfiI 0
TliI 0
TseI 0
Tsp45I 0
Tsp509I 0
TspMI 0
TspRI 0
Tth111I 0
XbaI 0
XcmI 0
XhoI 0
XmaI 0
XmnI 0
ZraI 0

Restriction Digest

One
Enzyme Cuts
None selected
Two
Enzyme Cuts
None selected
Three
Enzyme Cuts
None selected