pMCSG68

DNASU
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning

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Cutters
Show ORFs
Cutters
From: 222 To: 555 Length: 334bp Melting Temp: 63.1 °C GC Content: 55%
Name Type Location Length
pBR322 origin Bacterial Origin 3413 to 2825 589 bp
LIC Other 235 to 204 32 bp
T7 Promoter 400 to 384 17 bp
TEV Construction Suffix 243 to 223 21 bp
LacI Regulatory Protein 1437 to 2396 960 bp
AmpR Antibiotic Resistance 4444 to 3584 861 bp
ileX Other 1134 to 983 152 bp
argU Other 853 to 467 387 bp
strep Signalling Peptide 273 to 250 24 bp
His Signalling Peptide 309 to 292 18 bp
lac operator Other 359 to 381 23 bp
T7 term Terminator 72 to 26 47 bp
- / - - Location: - Length: -

Manage Enzymes

Enzyme Cuts
AatII 0
Acc65I 0
AccI 0
AciI 0
AclI 0
AcuI 0
AfeI 0
AflII 0
AflIII 0
AgeI 0
AhdI 0
AleI 0
AluI 0
AlwI 0
AlwNI 0
ApaI 0
ApaLI 0
ApeKI 0
ApoI 0
AscI 0
AseI 0
AsiSI 0
AvaI 0
AvaII 0
AvrII 0
BaeGI 0
BaeI 0
BamHI 0
BanI 0
BanII 0
BbsI 0
BbvCI 0
BbvI 0
BccI 0
BceAI 0
BcgI 0
BciVI 0
BclI 0
BfaI 0
BfuAI 0
BfuCI 0
BglI 0
BglII 0
BlpI 0
BmgBI 0
BmrI 0
BmtI 0
BpmI 0
Bpu10I 0
BpuEI 0
BsaAI 0
BsaBI 0
BsaHI 0
BsaI 0
BsaJI 0
BsaWI 0
BsaXI 0
BseRI 0
BseYI 0
BsgI 0
BsiEI 0
BsiHKAI 0
BsiWI 0
BslI 0
BsmAI 0
BsmBI 0
BsmFI 0
BsmI 0
BsoBI 0
Bsp1286I 0
BspCNI 0
BspDI 0
BspEI 0
BspHI 0
BspMI 0
BspQI 0
BsrBI 0
BsrDI 0
BsrFI 0
BsrGI 0
BsrI 0
BssHII 0
BssKI 0
BssSI 0
BstAPI 0
BstBI 0
BstEII 0
BstNI 0
BstUI 0
BstXI 0
BstYI 0
BstZ17I 0
Bsu36I 0
BtgI 0
BtgZI 0
BtsCI 0
BtsI 0
Cac8I 0
ClaI 0
CspCI 0
CviAII 0
CviKI_1 0
CviQI 0
DdeI 0
DpnI 0
DpnII 0
DraI 0
DraIII 0
DrdI 0
EaeI 0
EagI 0
EarI 0
EciI 0
Eco53kI 0
EcoNI 0
EcoO109I 0
EcoRI 0
EcoRV 0
FatI 0
FauI 0
Fnu4HI 0
FokI 0
FseI 0
FspI 0
HaeII 0
HaeIII 0
HgaI 0
HhaI 0
HinP1I 0
HincII 0
HindIII 0
HinfI 0
HpaI 0
HpaII 0
HphI 0
Hpy166II 0
Hpy188I 0
Hpy188III 0
Hpy99I 0
HpyAV 0
HpyCH4III 0
HpyCH4IV 0
HpyCH4V 0
KasI 0
KpnI 0
MboI 0
MboII 0
MfeI 0
MluI 0
MlyI 0
MmeI 0
MnlI 0
MscI 0
MseI 0
MslI 0
MspA1I 0
MspI 0
MwoI 0
NaeI 0
NarI 0
NciI 0
NcoI 0
NdeI 0
NgoMIV 0
NheI 0
NlaIII 0
NlaIV 0
NmeAIII 0
NotI 0
NruI 0
NsiI 0
NspI 0
PacI 0
PaeR7I 0
PciI 0
PflFI 0
PflMI 0
PhoI 0
PleI 0
PmeI 0
PmlI 0
PpuMI 0
PshAI 0
PsiI 0
PspGI 0
PspOMI 0
PspXI 0
PstI 0
PvuI 0
PvuII 0
RsaI 0
RsrII 0
SacI 0
SacII 0
SalI 0
SapI 0
Sau3AI 0
Sau96I 0
SbfI 0
ScaI 0
ScrFI 0
SexAI 0
SfaNI 0
SfcI 0
SfiI 0
SfoI 0
SgrAI 0
SmaI 0
SmlI 0
SnaBI 0
SpeI 0
SphI 0
SspI 0
StuI 0
StyD4I 0
StyI 0
SwaI 0
TaqI 0
TfiI 0
TliI 0
TseI 0
Tsp45I 0
Tsp509I 0
TspMI 0
TspRI 0
Tth111I 0
XbaI 0
XcmI 0
XhoI 0
XmaI 0
XmnI 0
ZraI 0

Restriction Digest

One
Enzyme Cuts
None selected
Two
Enzyme Cuts
None selected
Three
Enzyme Cuts
None selected