pRSF-BAD

DNASU
ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;

Map Viewer

Cutters
Show ORFs
Cutters
From: 222 To: 555 Length: 334bp Melting Temp: 63.1 °C GC Content: 55%
Name Type Location Length
-10 RNA I Other 3420 to 3425 6 bp
-10 AP Other 2783 to 2777 7 bp
-35 RNA I Other 3396 to 3401 6 bp
-35 AP Other 2805 to 2800 6 bp
Kan-R CDS 2735 to 1920 816 bp
araC CDS 987 to 109 879 bp
Operator O2 Other 1016 to 1033 18 bp
Operator O1 Other 1174 to 1195 22 bp
CAP site Other 1217 to 1230 14 bp
Operator I1 and I2 Other 1226 to 1264 39 bp
RBS Other 1318 to 1327 10 bp
ligation junction Xbal Other 3595 to 3600 6 bp
ligation junction Pacl Other 1606 to 1613 8 bp
RNA II Other 3541 to 2843 699 bp
RNA I Other 3432 to 3538 107 bp
RBS-reverse-weak RBS 2741 to 2749 9 bp
T7 Terminator Terminator 1643 to 1690 48 bp
rrnB T1 T2 Terminator 1356 to 1587 232 bp
T7TER primer (#35) Other 1647 to 1629 19 bp
BADT1A Primer Other 373 to 392 20 bp
BADT1B Primer Other 830 to 849 20 bp
ARAC3F primer (#145) Other 138 to 118 21 bp
ARAC5R primer (#148) Other 964 to 983 20 bp
wrong primer #62 off by 3 nt Other 1275 to 1296 22 bp
KAN1F primer (#120) Other 1950 to 1929 22 bp
araC promoter Promoter 1166 to 1138 29 bp
arabinose BAD promoter Promoter 1263 to 1290 28 bp
RSF ori - RSF1030 Homology Origin of Repication 2843 to 3592 750 bp
- / - - Location: - Length: -

Manage Enzymes

Enzyme Cuts
AatII 0
Acc65I 0
AccI 0
AciI 0
AclI 0
AcuI 0
AfeI 0
AflII 0
AflIII 0
AgeI 0
AhdI 0
AleI 0
AluI 0
AlwI 0
AlwNI 0
ApaI 0
ApaLI 0
ApeKI 0
ApoI 0
AscI 0
AseI 0
AsiSI 0
AvaI 0
AvaII 0
AvrII 0
BaeGI 0
BaeI 0
BamHI 0
BanI 0
BanII 0
BbsI 0
BbvCI 0
BbvI 0
BccI 0
BceAI 0
BcgI 0
BciVI 0
BclI 0
BfaI 0
BfuAI 0
BfuCI 0
BglI 0
BglII 0
BlpI 0
BmgBI 0
BmrI 0
BmtI 0
BpmI 0
Bpu10I 0
BpuEI 0
BsaAI 0
BsaBI 0
BsaHI 0
BsaI 0
BsaJI 0
BsaWI 0
BsaXI 0
BseRI 0
BseYI 0
BsgI 0
BsiEI 0
BsiHKAI 0
BsiWI 0
BslI 0
BsmAI 0
BsmBI 0
BsmFI 0
BsmI 0
BsoBI 0
Bsp1286I 0
BspCNI 0
BspDI 0
BspEI 0
BspHI 0
BspMI 0
BspQI 0
BsrBI 0
BsrDI 0
BsrFI 0
BsrGI 0
BsrI 0
BssHII 0
BssKI 0
BssSI 0
BstAPI 0
BstBI 0
BstEII 0
BstNI 0
BstUI 0
BstXI 0
BstYI 0
BstZ17I 0
Bsu36I 0
BtgI 0
BtgZI 0
BtsCI 0
BtsI 0
Cac8I 0
ClaI 0
CspCI 0
CviAII 0
CviKI_1 0
CviQI 0
DdeI 0
DpnI 0
DpnII 0
DraI 0
DraIII 0
DrdI 0
EaeI 0
EagI 0
EarI 0
EciI 0
Eco53kI 0
EcoNI 0
EcoO109I 0
EcoRI 0
EcoRV 0
FatI 0
FauI 0
Fnu4HI 0
FokI 0
FseI 0
FspI 0
HaeII 0
HaeIII 0
HgaI 0
HhaI 0
HinP1I 0
HincII 0
HindIII 0
HinfI 0
HpaI 0
HpaII 0
HphI 0
Hpy166II 0
Hpy188I 0
Hpy188III 0
Hpy99I 0
HpyAV 0
HpyCH4III 0
HpyCH4IV 0
HpyCH4V 0
KasI 0
KpnI 0
MboI 0
MboII 0
MfeI 0
MluI 0
MlyI 0
MmeI 0
MnlI 0
MscI 0
MseI 0
MslI 0
MspA1I 0
MspI 0
MwoI 0
NaeI 0
NarI 0
NciI 0
NcoI 0
NdeI 0
NgoMIV 0
NheI 0
NlaIII 0
NlaIV 0
NmeAIII 0
NotI 0
NruI 0
NsiI 0
NspI 0
PacI 0
PaeR7I 0
PciI 0
PflFI 0
PflMI 0
PhoI 0
PleI 0
PmeI 0
PmlI 0
PpuMI 0
PshAI 0
PsiI 0
PspGI 0
PspOMI 0
PspXI 0
PstI 0
PvuI 0
PvuII 0
RsaI 0
RsrII 0
SacI 0
SacII 0
SalI 0
SapI 0
Sau3AI 0
Sau96I 0
SbfI 0
ScaI 0
ScrFI 0
SexAI 0
SfaNI 0
SfcI 0
SfiI 0
SfoI 0
SgrAI 0
SmaI 0
SmlI 0
SnaBI 0
SpeI 0
SphI 0
SspI 0
StuI 0
StyD4I 0
StyI 0
SwaI 0
TaqI 0
TfiI 0
TliI 0
TseI 0
Tsp45I 0
Tsp509I 0
TspMI 0
TspRI 0
Tth111I 0
XbaI 0
XcmI 0
XhoI 0
XmaI 0
XmnI 0
ZraI 0

Restriction Digest

One
Enzyme Cuts
None selected
Two
Enzyme Cuts
None selected
Three
Enzyme Cuts
None selected